The "signal wire" as you call it is to tell the DC-to-DC that you are running a "Smart" Alternator via the ACC connection. You are not, so you do not want that connected.
EDIT 9:29AM
I see Scalf77 was posting while I was composing and he clarified the operation better than the manual and after rereading i need to correct what i posted earlier by just greying out my erroneous statements.
The manual is a little confusing but it seems that the Start Bat to Aux Bat charging portion of the DC to DC will not charge unless the ACC wire is high.
The AP40DC describes an ACC line which is really the +12V RUN signal rather than a having the ignition key in the accessory positon which is when the engine is not running.
So the AP40DC ACC wire is essentially the D+ wire on the Renogy DC-DC charger which can be controlled by the wires to the smart alternator, or just +12V RUN.
For a 40 amp DC to DC the standard recommendation is 6 AWG wire for up to about 10-15 feet. This will restrict the Start battery current to 50 amps in order to deliver 40 amps to the aux battery.
If connecting between batteries, standard practice is to put a fuse as close to the batteries as practical. However, with my 1997-2011 Econolines, the battery is already separated by fusing from the alternator so you can connect the DC to DC directly to the alternator (using a local mounting bolt for ground)without a fuse and the 6 AWG cabling.
In my 2002 E-250, I mounted a 5K BTU 12V Rooftop AC along with a pair of 150 amp-hr AGM's. The AC is connected directly to the AUX battery pair along with a 120VAC invertor. I had trouble meeting the demand for the AGM charging and 12V rooftop A/C demand. It seems I have it solved right now by dropping the DC-DC chargers completely and just using a 120Amp battery combiner relay. Victron also makes a lithium version.
https://www.amazon.com/gp/product/B071Y7C9MM/ref=ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1
The alternator is rated at 300 amps and was able to deliver 80-100+ amps to the Aux battery/AC combination as well as 15 amps to the vehicle while maintaining 14+ volts at idle. After about 5 minutes of running, the alternate got warm but not excessively. I did not have my IR gun handy so no temperatures are available till I get setup again. However, the alternator is smart in some respects.
This Is Truly A High Amp Premium Quality Well Built Alternator
It Has OE compliant functions : current limiting, thermal shutdown,
load dump protection, shorted field & lamp circuit protection
It Has OE compliant functions : current limiting, thermal shutdown,
load dump protection, shorted field & lamp circuit protection
https://www.ebay.com/itm/202513986004
This vendor has other alternators for different model years but I have not found one for the 1992 E-250 4.9L or my own 1990 F-150 4.9L.
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